pSrRGECO1
(Plasmid
#222940)
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PurposeExpresses RGECO1 (fluorescent calcium indicator) and pac (puromycin resistance) in the Choanoflagellate, Salpingoeca rosetta
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Depositing Lab
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Depositing OrganizationUniversity of Bergen
Located in Norway -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 222940 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 222940-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 222940-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneNK802
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Backbone manufacturerNicole King
- Backbone size w/o insert (bp) 6293
- Total vector size (bp) 6833
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Modifications to backbonemCherry sequence removed (714bp)
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Vector typeSalpingoeca rosetta expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRGECO1
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SpeciesSynthetic
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Insert Size (bp)1253
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.06.19.599169 for bioRxiv preprint.
DNA (Catalog # 222940-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 222940-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSrRGECO1 was a gift from Pawel Burkhardt (Addgene plasmid # 222940 ; http://n2t.net/addgene:222940 ; RRID:Addgene_222940) -
For your References section:
Electrical signaling and coordinated behavior in the closest relative of animals. Colgren J, Burkhardt P. Sci Adv. 2025 Jan 10;11(2):eadr7434. doi: 10.1126/sciadv.adr7434. Epub 2025 Jan 8. 10.1126/sciadv.adr7434 PubMed 39772683
Map uploaded by the depositor.
