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SRS-MTBD(22/19), AB(monomer), wt
(Plasmid #22380)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22380 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET42a
  • Backbone manufacturer
    EMD
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    6634
  • GenBank ID
    FJ039896
  • Tags / Fusion Proteins
    • myc (C terminal on insert)
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SRS-MTBD(22/19), AB(monomer), wt was a gift from Ron Vale (Addgene plasmid # 22380 ; http://n2t.net/addgene:22380 ; RRID:Addgene_22380)
  • For your References section:

    The affinity of the dynein microtubule-binding domain is modulated by the conformation of its coiled-coil stalk. Gibbons IR, Garbarino JE, Tan CE, Reck-Peterson SL, Vale RD, Carter AP. J Biol Chem. 2005 Jun 24. 280(25):23960-5. 10.1074/jbc.M501636200 PubMed 15826937