Skip to main content
Addgene
Open Menu

pubc-FRB-BFP-IRES-FKBP-HaloTag_tdMCP-NLS-HA (RIDR negative control)
(Plasmid #224133)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224133 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart

Backbone

  • Vector backbone
    phage ubc
  • Backbone size w/o insert (bp) 6251
  • Total vector size (bp) 9988
  • Vector type
    Mammalian Expression
  • Selectable markers
    HaloTag

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pubc-FRB-tagBFP-IRES-FKBP-HaloTag_tdMCP-NLS-HA
  • Alt name
    RIDR negative control
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3737
  • Promoter ubc
  • Tags / Fusion Proteins
    • FRB-tagBFP (N terminal on insert)
    • FKBP-HaloTag-tdMCP-NLS-HA (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pubc-FRB-BFP-IRES-FKBP-HaloTag_tdMCP-NLS-HA (RIDR negative control) was a gift from Bin Wu (Addgene plasmid # 224133 ; http://n2t.net/addgene:224133 ; RRID:Addgene_224133)

  • For your References section:

    A rapid inducible RNA decay system reveals fast mRNA decay in P-bodies. Blake LA, Watkins L, Liu Y, Inoue T, Wu B. Nat Commun. 2024 Mar 28;15(1):2720. doi: 10.1038/s41467-024-46943-z. 10.1038/s41467-024-46943-z PubMed 38548718
Related items: