This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

SFG.CNa22_opt.IRES.eGFP
(Plasmid #22491)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22491 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Codon optimised Calcineurin A mutant 22
  • Alt name
    CNa22
  • Alt name
    CNa
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1566
  • Mutation
    V314R; Y341F
  • GenBank ID
    GQ463595
  • Entrez Gene
    PPP3CA (a.k.a. ACCIID, CALN, CALNA, CALNA1, CCN1, CNA1, IECEE, IECEE1, PPP2B)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site MluI (not destroyed)
  • 5′ sequencing primer ttacacagtcctgctgaccacc
  • 3′ sequencing primer caagcggcttcggccagtaac
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SFG.CNa22_opt.IRES.eGFP was a gift from Martin Pule (Addgene plasmid # 22491 ; http://n2t.net/addgene:22491 ; RRID:Addgene_22491)
  • For your References section:

    Generation of EBV-specific cytotoxic T-cells that are resistant to calcineurin inhibitors for the treatment of post-transplant lymphoproliferative disease. Brewin J, Mancao C, Straathof K, Karlsson H, Samarasinghe S, Amrolia PJ, Pule M. Blood. 2009 Sep 21. ():. 10.1182/blood-2009-07-228387 PubMed 19770360