ADRM1_MTT_Del_pGEX-4T-1
(Plasmid #225429)

• Purpose: Expresses a truncated construct of hRpn13 Pru having deletion at the N-terminus
• Depositing Lab: Kylie Walters
• Publication: Hassan et al Nat Commun. 2025 Jul 1;16(1):5669. doi: 10.1038/s41467-025-60843-w.

Backbone

• Vector backbone: pGEX-4T-1
• Backbone manufacturer: Genscript
• Total vector size (bp): 5392
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: RPN13
• Species: H. sapiens (human)
• Insert Size (bp): 882
• Mutation: deleted amino acid 1-3
• GenBank ID: NM_175573
• Entrez Gene: ADRM1 (a.k.a. ARM-1, ARM1, GP110, PSMD16)
• Tag / Fusion Protein:
  • GST (N terminal on insert) (N terminal on backbone)

Cloning Information

• Cloning method: Gene Synthesis
• 5′ sequencing primer: pGEX Fwd

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This clone was synthesized by using Custom Gene Services from Genscript

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry