An adaptive peptide-binding site in ubiquitin receptor hRpn13 revealed by structural studies.
Hassan B, Chandravanshi M, Ng MY, Negi H, Wilson BAP, Walters KJ
Nat Commun. 2025 Jul 1;16(1):5669. doi: 10.1038/s41467-025-60843-w.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 225429 | ADRM1_MTT_Del_pGEX-4T-1 | Expresses a truncated construct of hRpn13 Pru having deletion at the N-terminus |
| 237412 | pET_3a_Ub_ENT | Expresses Ubiquitin with a disordered tail from the extreme N terminus of Rpn13 |
| 237413 | pET_3a_Ub_EDGEGEE | Expresses Ubiquitin with a disordered tail with an acidic sequence |
| 237414 | ADRM1_MTT_Del_pGEX-4T-1 with Stop Codon | Expresses hRpn13 without the first three residues (MTT) of the native sequence |