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pET_3a_Ub_ENT
(Plasmid #237412)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 237412 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET-3A
  • Backbone size w/o insert (bp) 4595
  • Total vector size (bp) 4874
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ubiquitin_ENT
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    279
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Genscript

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET_3a_Ub_ENT was a gift from Kylie Walters (Addgene plasmid # 237412 ; http://n2t.net/addgene:237412 ; RRID:Addgene_237412)

  • For your References section:

    An adaptive peptide-binding site in ubiquitin receptor hRpn13 revealed by structural studies. Hassan B, Chandravanshi M, Ng MY, Negi H, Wilson BAP, Walters KJ. Nat Commun. 2025 Jul 1;16(1):5669. doi: 10.1038/s41467-025-60843-w. 10.1038/s41467-025-60843-w PubMed 40595513
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