pMC_HT7_BSDminus_F0
(Plasmid
#225627)
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PurposeDonor cassette plasmid for high-throughput tagging, encoding an HaloTag7 synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 225627 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepMC.BESPX
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Vector typeCRISPR
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHT7 and BSD
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Alt nameHaloTag7
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Alt nameBlasticidin-S deaminase
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SpeciesSynthetic; Aspergillus terreus
Cloning Information
- Cloning method Unknown
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMC_HT7_BSDminus_F0 was a gift from Ophir Shalem (Addgene plasmid # 225627 ; http://n2t.net/addgene:225627 ; RRID:Addgene_225627)
