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pJMP10233_pCRISPRt-H
(Plasmid #225832)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225832 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    R6K_
  • Vector type
    Bacterial Expression, Synthetic Biology ; CRISPR Associated Transposition (CAST)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BW25141
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Cas678, TniQ, TnsABC
  • Species
    Vibrio cholera

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The selectable marker is used when selecting for transposition into a bacterium. Distributed in E. coli BW25141 (a pir+ strain). Please visit https://doi.org/10.1101/2024.03.01.582922 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJMP10233_pCRISPRt-H was a gift from Jason Peters (Addgene plasmid # 225832 ; http://n2t.net/addgene:225832 ; RRID:Addgene_225832)

  • For your References section:

    A Targeted Genome-scale Overexpression Platform for Proteobacteria. Banta AB, Hall AN, Myers KS, Ward RD, Davis BC, Cuellar RA, Place M, Freeh CC, Bacon EE, Peters JM. bioRxiv 2024.03.01.582922 10.1101/2024.03.01.582922
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