A Targeted Genome-scale Overexpression Platform for Proteobacteria
Banta AB, Hall AN, Myers KS, Ward RD, Davis BC, Cuellar RA, Place M, Freeh CC, Bacon EE, Peters JM
bioRxiv 2024.03.01.582922
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 225832 | pJMP10233_pCRISPRt-H | CRISPRt helper plasmid to construct CRISPRt and CRISPRtOE strains. Cas678, TniQ, TnsABC expression. |
| 225833 | pJMP10395_pCRISPRt-T-gen | Tn6677 transposon with guide RNA (BsaI site) and gm resistance expression. |
| 225834 | pJMP10608_pCRISPRt-T-gen-T03 | Tn6677 transposon with guide RNA (mscar T03 spacer) and gm resistance expression. |
| 225835 | pJMP10397_pCRISPRt-T-kan | Tn6677 transposon with guide RNA (BsaI site) and kan resistance expression. |
| 225836 | pJMP10611_pCRISPRt-T-kan-T03 | Tn6677 transposon with guide RNA (mscar T03 spacer) and kan resistance expression. |
| 225837 | pJMP10621_pCRISPRt-T-apr | Tn6677 transposon with guide RNA (BsaI site) and apr resistance expression. |
| 225838 | pJMP10745_pCRISPRt-T-apr-T03 | Tn6677 transposon with guide RNA (mscar T03 spacer) and apr resistance expression. |
| 225839 | pJMP10746_pCRISPRt-T-spec | Tn6677 transposon with guide RNA (BsaI site) and spec resistance expression. |
| 225840 | pJMP10747_pCRISPRt-T-spec-T03 | Tn6677 transposon with guide RNA (mscar T03 spacer) and spec resistance expression. |
| 225841 | pJMP10748_pCRISPRt-T-cm | Tn6677 transposon with guide RNA (BsaI site) and cm resistance expression. |
| 225842 | pJMP10749_pCRISPRt-T-cm-T03 | Tn6677 transposon with guide RNA (mscar T03 spacer) and cm resistance expression. |
| 225843 | pJMP10750_pCRISPRt-T-hyg | Tn6677 transposon with guide RNA (BsaI site) and hyg resistance expression. |
| 225844 | pJMP10751_pCRISPRt-T-hyg-T03 | Tn6677 transposon with guide RNA (mscar T03 spacer) and hyg resistance expression. |
| 225845 | pJMP10471_pCRISPRtOE-noP-gen | Tn6677 transposon with guide RNA (BsaI site) and gm resistance expression; no promoter. |
| 225846 | pJMP10474_pCRISPRtOE-PH-gen | Tn6677 transposon with guide RNA (BsaI site) and gm resistance expression; promoter H. |
| 225847 | pJMP10541_pCRISPRtOE-PH-gen-LZ1 | Tn6677 transposon with guide RNA (LZ1 guide) and gm resistance expression; promoter H. |
| 225848 | pJMP10476_pCRISPRtOE-noP-kan | Tn6677 transposon with guide RNA (BsaI site) and kan resistance expression; no promoter. |
| 225849 | pJMP10477_pCRISPRtOE-PA-kan | Tn6677 transposon with guide RNA (BsaI site) and kan resistance expression; promoter A. |
| 225850 | pJMP10478_pCRISPRtOE-PH-kan | Tn6677 transposon with guide RNA (BsaI site) and kan resistance expression; promoter H. |
| 225851 | pJMP10479_pCRISPRtOE-PV-kan | Tn6677 transposon with guide RNA (BsaI site) and kan resistance expression; promoter V. |
| 225852 | pJMP10492_pCRISPRtOE-PH-kan-LZ1 | Tn6677 transposon with guide RNA (LZ1 guide) and kan resistance expression; promoter H. |
| 225853 | pJMP15019_pCRISPRtOE-noP-apr | Tn6677 transposon with guide RNA (BsaI site) and apr resistance expression; no promoter. |
| 225854 | pJMP10622_pCRISPRtOE-PH-apr | Tn6677 transposon with guide RNA (BsaI site) and apr resistance expression; promoter H. |
| 225855 | pJMP10624_pCRISPRtOE-PH-apr-LZ1 | Tn6677 transposon with guide RNA (LZ1 guide) and apr resistance expression; promoter H. |
| 225856 | pJMP10996_pCRISPRtOE-noP-cm | Tn6677 transposon with guide RNA (BsaI site) and cm resistance expression; no promoter. |
| 225857 | pJMP10998_pCRISPRtOE-PH-cm | Tn6677 transposon with guide RNA (BsaI site) and cm resistance expression; promoter H. |
| 225858 | pJMP15023_pCRISPRtOE-PH-cm-LZ1 | Tn6677 transposon with guide RNA (LZ1 guide) and cm resistance expression; promoter H. |
| 225859 | pJMP15011_pCRISPRtOE-noP-hyg | Tn6677 transposon with guide RNA (BsaI site) and hyg resistance expression; no promoter. |
| 225860 | pJMP15014_pCRISPRtOE-PH-hyg | Tn6677 transposon with guide RNA (BsaI site) and hyg resistance expression; promoter H. |
| 225861 | pJMP15021_pCRISPRtOE-PH-hyg-LZ1 | Tn6677 transposon with guide RNA (LZ1 guide) and hyg resistance expression; promoter H. |
| 225862 | pJMP10180_pTn7-mscar-spec | Tn7 transposon plasmid with mScarlet-I and spec resistance expression. |
| 225863 | pJMP10239_pTn7-mscar-gen | Tn7 transposon plasmid with mScarlet-I and gen resistance expression. |
| 225864 | pJMP10245_pTn7-mscar-kan | Tn7 transposon plasmid with mScarlet-I and kan resistance expression. |
| 225865 | pJMP10301_pTn7-mscar-cm | Tn7 transposon plasmid with mScarlet-I and cm resistance expression. |
| 225866 | pJMP10400_pTn7-mscar-apr | Tn7 transposon plasmid with mScarlet-I and apr resistance expression. |
| 225867 | pJMP10206_pTn7-LP-mscar-spec | Tn7 transposon plasmid with landing pad-mScarlet-I for CRISPRtOE-mScarlet-I and spec resistance expression. |
| 225868 | pJMP10241_pTn7-LP-mscar-gen | Tn7 transposon plasmid with landing pad-mScarlet-I for CRISPRtOE-mScarlet-I and gen resistance expression. |
| 225869 | pJMP10247_pTn7-LP-mscar-kan | Tn7 transposon plasmid with landing pad-mScarlet-I for CRISPRtOE-mScarlet-I and kan resistance expression. |
| 225870 | pJMP10303_pTn7-LP-mscar-cm | Tn7 transposon plasmid with landing pad-mScarlet-I for CRISPRtOE-mScarlet-I and cm resistance expression. |
| 225871 | pJMP10402_pTn7-LP-mscar-apr | Tn7 transposon plasmid with landing pad-mScarlet-I for CRISPRtOE-mScarlet-I and apr resistance expression. |