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pJMP10477_pCRISPRtOE-PA-kan
(Plasmid #225849)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225849 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    R6K_
  • Vector type
    Bacterial Expression, Synthetic Biology ; CRISPR Associated Transposition (CAST)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BW25141
  • Growth instructions
    Plasmid contains both Ampicillin (backbone) and Kanamycin (transposon) resistance genes. Either antibiotic can be used for plasmid growth and propagation but for selection of downstream transposition events Kanamycin must be used.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CRISPRtOE transposon with gRNA expression cassette (no guide) and synthetic promoter A
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.03.01.582922 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJMP10477_pCRISPRtOE-PA-kan was a gift from Jason Peters (Addgene plasmid # 225849 ; http://n2t.net/addgene:225849 ; RRID:Addgene_225849)

  • For your References section:

    A Targeted Genome-scale Overexpression Platform for Proteobacteria. Banta AB, Hall AN, Myers KS, Ward RD, Davis BC, Cuellar RA, Place M, Freeh CC, Bacon EE, Peters JM. bioRxiv 2024.03.01.582922 10.1101/2024.03.01.582922
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