pcDNA3.1(-).TagRFP.T-2A-V5-Tau2N4R-WPRE
(Plasmid
#226381)
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PurposeExpresses tau propagation ORF with V5 tag on WT 2N4R tau
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Depositing Lab
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Depositing OrganizationMassachusetts General Hospital
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 226381 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 226381-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 226381-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA3.1(-)
- Backbone size w/o insert (bp) 5427
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTau2N4R
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SpeciesH. sapiens (human); synthetic
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Insert Size (bp)2174
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Entrez GeneMAPT (a.k.a. DDPAC, FTD1, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40)
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Tag
/ Fusion Protein
- TagRFP.T.2A.V5 (N terminal on insert)
Cloning Information
- Cloning method Other
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 226381-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 226381-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1(-).TagRFP.T-2A-V5-Tau2N4R-WPRE was a gift from Bradley Hyman (Addgene plasmid # 226381 ; http://n2t.net/addgene:226381 ; RRID:Addgene_226381) -
For your References section:
Alzheimer disease-associated tau post-translational modification mimics impact tau propagation and uptake. Dickson JR, Sobolewski RGR, Fernandes AR, Cooper JM, Fan Z, Chung M, Donahue C, Oakley DH, Strickland DK, Hyman BT. J Neuropathol Exp Neurol. 2025 Jun 1;84(6):459-470. doi: 10.1093/jnen/nlaf007. 10.1093/jnen/nlaf007 PubMed 39984820
Ready-to-use antibodies
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