pDL1403
(Plasmid
#226635)
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PurposeThis plasmid induces natural competence in classical biotype Vibrio cholerae and expresses the genes tfox and qstR.
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Depositing Lab
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Depositing OrganizationTufts University
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 226635 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 226635-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 226635-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepMMB67EH
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Pir1
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nametfox
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SpeciesVibrio cholerae
Gene/Insert 2
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Gene/Insert nameqstR
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SpeciesVibrio cholerae
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.06.17.599363 for bioRxiv preprint.
DNA (Catalog # 226635-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 226635-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDL1403 was a gift from Andrew Camilli (Addgene plasmid # 226635 ; http://n2t.net/addgene:226635 ; RRID:Addgene_226635) -
For your References section:
A Vibrio cholerae anti-phage system depletes nicotinamide adenine dinucleotide to restrict virulent bacteriophages. Woldetsadik YA, Lazinski DW, Camilli A. mBio. 2024 Oct 8:e0245724. doi: 10.1128/mbio.02457-24. 10.1128/mbio.02457-24 PubMed 39377576
Map uploaded by the depositor.
