pLostfoX-Kan
(Plasmid #99865)

• Purpose: TfoX induction vector for chitin-pathway transformation (KanR)
• Depositing Lab: Mark Mandel
• Publication: Brooks JF et al Proc Natl Acad Sci U S A. 2014 Dec 2;111(48):17284-9. doi: 10.1073/pnas.1415957111. Epub 2014 Nov 17.

Backbone

• Vector backbone: pEVS79
• Backbone manufacturer: Feliciano, B. Masters Thesis. Honolulu: University of Hawaii; 2000. "The identification and characterization of a Vibrio..."
• Modifications to backbone: pLostfoX was created by cloning 995 bp of V. fischeri ES114 DNA containing the tfoXVF ORF into pEVS79; pLostfoX-Kan was created by replacing the chloramphenicol resistance of pLostfoX with a kanamycin resistance via a SphI restriction site which has been introduced with PCR using Primers containing SphI sites. Additional reference for pREVS79: Stabb EV, Ruby EG. 2002. RP4-based plasmids for conjugation between Escherichia coli and members of the Vibrionaceae. Methods Enzymol. 358:413–426
• Vector type: V. fischeri suicide cloning vector, CmR, TcR

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: 50 μg/mL in LB; plasmid has additional Tetracyclin resistance
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: tfoXVF
• Alt name: sxy
• Species: Vibrio fischeri
• Insert Size (bp): 979
• GenBank ID: VF_0896

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry