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pLostfoX-Kan
(Plasmid #99865)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 99865 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEVS79
  • Backbone manufacturer
    Feliciano, B. Masters Thesis. Honolulu: University of Hawaii; 2000. "The identification and characterization of a Vibrio..."
  • Modifications to backbone
    pLostfoX was created by cloning 995 bp of V. fischeri ES114 DNA containing the tfoXVF ORF into pEVS79; pLostfoX-Kan was created by replacing the chloramphenicol resistance of pLostfoX with a kanamycin resistance via a SphI restriction site which has been introduced with PCR using Primers containing SphI sites. Additional reference for pREVS79: Stabb EV, Ruby EG. 2002. RP4-based plasmids for conjugation between Escherichia coli and members of the Vibrionaceae. Methods Enzymol. 358:413–426
  • Vector type
    V. fischeri suicide cloning vector, CmR, TcR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    50 μg/mL in LB; plasmid has additional Tetracyclin resistance
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    tfoXVF
  • Alt name
    sxy
  • Species
    Vibrio fischeri
  • Insert Size (bp)
    979
  • GenBank ID
    VF_0896

Cloning Information

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLostfoX-Kan was a gift from Mark Mandel (Addgene plasmid # 99865 ; http://n2t.net/addgene:99865 ; RRID:Addgene_99865)
  • For your References section:

    Global discovery of colonization determinants in the squid symbiont Vibrio fischeri. Brooks JF 2nd, Gyllborg MC, Cronin DC, Quillin SJ, Mallama CA, Foxall R, Whistler C, Goodman AL, Mandel MJ. Proc Natl Acad Sci U S A. 2014 Dec 2;111(48):17284-9. doi: 10.1073/pnas.1415957111. Epub 2014 Nov 17. 10.1073/pnas.1415957111 PubMed 25404340