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pMarVF1
(Plasmid #99579)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 99579 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSAM
  • Backbone manufacturer
    Jeffrey I. Gordon, PMID: 19748469
  • Backbone size w/o insert (bp) 3608
  • Total vector size (bp) 4552
  • Modifications to backbone
    nrdR promoter was added using BamHI/NdeI sites to drive expression of himar1C9 transposase
  • Vector type
    Suicide vector in V. fischeri ES114
  • Selectable markers
    Erythromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    S17-1 λpir
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    V. fischeri mariner transposon (with erythromycin-resistance (ErmR) cassette)
  • Insert Size (bp)
    944

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer Unknown
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMarVF1 was a gift from Mark Mandel (Addgene plasmid # 99579 ; http://n2t.net/addgene:99579 ; RRID:Addgene_99579)
  • For your References section:

    Global discovery of colonization determinants in the squid symbiont Vibrio fischeri. Brooks JF 2nd, Gyllborg MC, Cronin DC, Quillin SJ, Mallama CA, Foxall R, Whistler C, Goodman AL, Mandel MJ. Proc Natl Acad Sci U S A. 2014 Dec 2;111(48):17284-9. doi: 10.1073/pnas.1415957111. Epub 2014 Nov 17. 10.1073/pnas.1415957111 PubMed 25404340