pMyt1a
(Plasmid #22695)

• Depositing Lab: Lynn Hudson
• Publication: Kim et al Mol Cell Biol. 1992 Dec . 12(12):5632-9.

Backbone

• Vector backbone: pT7T3
• Backbone manufacturer: Amersham
• Backbone size w/o insert (bp): 2883
• Vector type: Cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5 alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Human Myelin Transcription Factor 1
• Alt name: Myt1
• Species: H. sapiens (human)
• Insert Size (bp): 2200
• Entrez Gene: MYT1 (a.k.a. MTF1, MYTI, PLPB1, C20orf36)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T3 Promoter
• 3′ sequencing primer: T7 promoter

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: Human Myt1 cDNA was cloned in Hudson lab

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This Myt1 DNA was screened from a fetal brain expression library. It contains the carboxy but not the amino terminus of the protein. It is a partial cDNA