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(Plasmid #22707)


Item Catalog # Description Quantity Price (USD)
Plasmid 22707 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5348
  • Vector type
    Drosophila expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Dh5 alpha and XL-1 blue
  • Copy number
    High Copy


  • Gene/Insert name
    micro RNA 279
  • Alt name
  • Species
    D. melanogaster (fly); primary transcript
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    mir-279 (a.k.a. Dmel_CR33590, 0962/07, CR33590, Dmel\CR33590, MiR-279, Mir-279, dme-mir-279, l(3)S096207, miR, miR-279, miR279, mir-279a)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer Ac5 forward primer site
  • 3′ sequencing primer BGH reverse priming site
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAc5.1A-Dm-miR-279 was a gift from Elisa Izaurralde (Addgene plasmid # 22707 ; ; RRID:Addgene_22707)
  • For your References section:

    Target-specific requirements for enhancers of decapping in miRNA-mediated gene silencing. Eulalio A, Rehwinkel J, Stricker M, Huntzinger E, Yang SF, Doerks T, Dorner S, Bork P, Boutros M, Izaurralde E. Genes Dev. 2007 Oct 15. 21(20):2558-70. 10.1101/gad.443107 PubMed 17901217