pRC319
(Plasmid #61272)

• Purpose: Also referred to as pΦRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, kanamycin resistance cassette and f1 origin for packaging in M13 particles.
• Depositing Lab: Timothy Lu
• Publication: Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.1038/nbt.3011.

Backbone

• Vector backbone: pZE22G
• Backbone manufacturer: EXPRESSYS
• Backbone size w/o insert (bp): 2629
• Total vector size (bp): 7637
• Modifications to backbone: f1 origin
• Vector type: Bacterial Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: For creation of transducing particles, grow in a strain able to package plasmids containing f1 origin, such as one containing helper plasmid M13cp
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cas9, tracrRNA, crRNA
• Alt name: CRISPR-Cas
• Species: Streptococcus pyogenes
• Insert Size (bp): 5008
• Tag / Fusion Protein:
  • 6xHis (N terminal on insert)

Resource Information

• Supplemental Documents:
  • pΦRGNndm-1
• A portion of this plasmid was derived from a plasmid made by: Cas9 from pMJ806 (Addgene 39312)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry