pMM441
(Plasmid #61271)

• Purpose: Also referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.
• Depositing Lab: Timothy Lu
• Publication: Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.1038/nbt.3011.

Backbone

• Vector backbone: Custom pR1162-CmR
• Vector type: Bacterial Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Cas9, tracrRNA, crRNA
• Alt name: CRISPR-Cas
• Species: Streptococcus pyogenes
• Insert Size (bp): 5008
• Tag / Fusion Protein:
  • 6xHis (N terminal on insert)

Resource Information

• Supplemental Documents:
  • Annotated pMM441
• A portion of this plasmid was derived from a plasmid made by: The Cas9 enzyme was originally cloned from addgene plasmid 39312 (pMJ806). The tracrRNA and crRNA were synthesized.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry