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pTag5A-rPrmpMyc
(Plasmid #22734)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22734 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 22734-D50 50 μg of DNA in Tris buffer $495
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DNA 22734-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCMV-Tag 5A
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5 alpha. Grows under normal conditions 37°C, LB broth
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Proline-rich transmembrane protein
  • Alt name
    Prmp
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    618
  • Tag / Fusion Protein
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T3 Promoter
  • 3′ sequencing primer T7 promoter
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    DNA cloned in Hudson Laboratory

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

T

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTag5A-rPrmpMyc was a gift from Lynn Hudson (Addgene plasmid # 22734 ; http://n2t.net/addgene:22734 ; RRID:Addgene_22734)

  • For your References section:

    Identification of a novel oligodendrocyte cell adhesion protein using gene expression profiling. Nielsen JA, Maric D, Lau P, Barker JL, Hudson LD. J Neurosci. 2006 Sep 27. 26(39):9881-91. 10.1523/JNEUROSCI.2246-06.2006 PubMed 17005852
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