pk335-CARGO-6mer-70kb-DSF
(Plasmid
#227498)
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Purpose6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus
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Depositing Lab
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Depositing OrganizationStanford University
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 227498 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 227498-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 227498-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepk335-BFP
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Vector typeCRISPR
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Selectable markersNeomycin (select with G418) ; TagBFP
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCARGO to 70kb Downstream Fgf5
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Alt nameCARGO37
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gRNA/shRNA sequenceGGAAGCATCATCAGTGTGTT, AAATGCATCTTCTCGAATGC, ACACGTGGCCAAAGCATTTG, CCCCATTACCGAGAGAAACG, TGAACATATAGCATGTATAA, CTCATACACACCTATCTATA
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SpeciesM. musculus (mouse), Synthetic
Cloning Information
- Cloning method Other
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 227498-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 227498-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pk335-CARGO-6mer-70kb-DSF was a gift from Joanna Wysocka (Addgene plasmid # 227498 ; http://n2t.net/addgene:227498 ; RRID:Addgene_227498) -
For your References section:
Long-range regulation of transcription scales with genomic distance in a gene-specific manner. Jensen CL, Chen LF, Swigut T, Crocker OJ, Yao D, Bassik MC, Ferrell JE Jr, Boettiger AN, Wysocka J. Mol Cell. 2025 Jan 16;85(2):347-361.e7. doi: 10.1016/j.molcel.2024.10.021. Epub 2024 Dec 2. 10.1016/j.molcel.2024.10.021 PubMed 39626660
