myc-Orai1 E190A pHAGE
(Plasmid #22753)

• Depositing Lab: Anjana Rao
• Publication: Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

Backbone

• Vector backbone: pHAGE
• Backbone manufacturer: Gift of Richard Mulligan
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Orai1 E190A
• Alt name: Orai1
• Species: H. sapiens (human)
• Insert Size (bp): 1000
• Mutation: E190A
• Entrez Gene: ORAI1 (a.k.a. CRACM1, IMD9, ORAT1, TAM2, TMEM142A)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For lentiviral transduction, N-terminally myc-tagged versions of Orai1 were directly cloned NotI-BamHI into the bicistronic pHAGE-UBC-eGFP-IRES-eGFP vector (R. Mulligan, Harvard Medical School), replacing the first eGFP with myc-Orai1.

Lab Plasmid # SF214. The Author's sequence includes the ORF plus 60 nt of vector boundary 3' and 5'.