pJRTCLCrVA010
(Plasmid #22843)

• Depositing Lab: Niki Robertson
• Publication: Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

Backbone

• Vector backbone: pBlueScript II sk+(original backbone)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 2961
• Vector type: Bacterial Expression ; cotton expression vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: A DNA containing GFP
• Alt name: pJRT010 or pJRTCLCrVA.010
• Alt name: CLCrVA:smRSGFP
• Alt name: JRT010
• Species: Cotton Leaf Crumple Virus
• Insert Size (bp): 3106
• Mutation: Cotton Leaf Crumple Virus A component with Coat Protein (AR1 gene) deleted and replaced with a multiple cloning site. A smRSGFP has been inserted in frame and is driven by the viral coat protein promoter.

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HincII (destroyed during cloning)
• 3′ cloning site: SacI (not destroyed)
• 5′ sequencing primer: M13R
• 3′ sequencing primer: M13F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The wild-type A component was provided by Dr. Judith K. Brown and Dr. Ali M. Idris from the University of Arizona, Tucson.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The smRSGFP used is Genbank Accession U70496 (Davis and Vierstra, 1998).

Please note that the actual sequence is slightly different than the map below (which is based on the author's sequence). An A->G mutation eliminates the NruI site.

--Must be bombarded with appropriate B DNA for systemic infection