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pCph8
(Plasmid #22869)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22869 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pPROTet
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 2021
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    EnvZ knockout strain
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pCph8
  • Species
    Synechocystis/E.coli fusion gene
  • Insert Size (bp)
    2236
  • Mutation
    First 517 amino acids of Cph1 and the last 229 amino acids of EnvZ. ILE TO VAL mutation at position 136 of the HK EnvZ domain.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn1 (not destroyed)
  • 3′ cloning site Xba1 (not destroyed)
  • 5′ sequencing primer sequence
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

This plasmid was also used in the 2009 Cell paper: "A synthetic genetic edge detection program." PMID:19563759

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCph8 was a gift from Christopher Voigt (Addgene plasmid # 22869 ; http://n2t.net/addgene:22869 ; RRID:Addgene_22869)
  • For your References section:

    Synthetic biology: engineering Escherichia coli to see light. Levskaya A, Chevalier AA, Tabor JJ, Simpson ZB, Lavery LA, Levy M, Davidson EA, Scouras A, Ellington AD, Marcotte EM, Voigt CA. Nature. 2005 Nov 24. 438(7067):441-2. 10.1038/nature04405 PubMed 16306980