pCph8
(Plasmid #22869)

• Depositing Lab: Christopher Voigt
• Publication: Levskaya et al Nature. 2005 Nov 24. 438(7067):441-2.

Backbone

• Vector backbone: pPROTet
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 2021
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: EnvZ knockout strain
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pCph8
• Species: Synechocystis/E.coli fusion gene
• Insert Size (bp): 2236
• Mutation: First 517 amino acids of Cph1 and the last 229 amino acids of EnvZ. ILE TO VAL mutation at position 136 of the HK EnvZ domain.

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn1 (not destroyed)
• 3′ cloning site: Xba1 (not destroyed)
• 5′ sequencing primer: sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was also used in the 2009 Cell paper: "A synthetic genetic edge detection program." PMID:19563759