|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22869||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2021
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
Growth instructionsEnvZ knockout strain
Copy numberHigh Copy
SpeciesSynechocystis/E.coli fusion gene
Insert Size (bp)2236
MutationFirst 517 amino acids of Cph1 and the last 229 amino acids of EnvZ. ILE TO VAL mutation at position 136 of the HK EnvZ domain.
- Cloning method Restriction Enzyme
- 5′ cloning site Kpn1 (not destroyed)
- 3′ cloning site Xba1 (not destroyed)
- 5′ sequencing primer sequence (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This plasmid was also used in the 2009 Cell paper: "A synthetic genetic edge detection program." PMID:19563759
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCph8 was a gift from Christopher Voigt (Addgene plasmid # 22869 ; http://n2t.net/addgene:22869 ; RRID:Addgene_22869)
For your References section:Synthetic biology: engineering Escherichia coli to see light. Levskaya A, Chevalier AA, Tabor JJ, Simpson ZB, Lavery LA, Levy M, Davidson EA, Scouras A, Ellington AD, Marcotte EM, Voigt CA. Nature. 2005 Nov 24. 438(7067):441-2. 10.1038/nature04405 PubMed 16306980