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pAcBac1-EcTyr-TAG
(Plasmid #228782)

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This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 228782 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 228782-D50 50 μg of DNA in Tris buffer $495
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DNA 228782-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pAcBac1
  • Total vector size (bp) 12860
  • Vector type
    Mammalian Expression ; Baculoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    EcTyrRS
  • Alt name
    EcYRS
  • Alt name
    E. coli tyrosyl synthetase
  • Species
    E. coli

Gene/Insert 2

  • Gene/Insert name
    EcYtR
  • Species
    E. coli
  • Mutation
    TAG suppressor

Gene/Insert 3

  • Gene/Insert name
    BstR
  • Species
    Bacillus stearothermophilus
  • Mutation
    TAG suppressor

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAcBac1-EcTyr-TAG was a gift from Abhishek Chatterjee (Addgene plasmid # 228782 ; http://n2t.net/addgene:228782 ; RRID:Addgene_228782)

  • For your References section:

    An Efficient Opal-Suppressor Tryptophanyl Pair Creates New Routes for Simultaneously Incorporating up to Three Distinct Noncanonical Amino Acids into Proteins in Mammalian Cells. Osgood AO, Zheng Y, Roy SJS, Biris N, Hussain M, Loynd C, Jewel D, Italia JS, Chatterjee A. Angew Chem Int Ed Engl. 2023 May 2;62(19):e202219269. doi: 10.1002/anie.202219269. Epub 2023 Apr 12. 10.1002/anie.202219269 PubMed 36905325
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