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pGL3-p27 T187A Luc
(Plasmid #23048)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 23048 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5223
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    T187A: mutation at codon 187 changing from T (ACG) to A (GCG), additionally 3 silent mutations introducing a BamH I site at codons 182/183/184 (GGT TCT GTG) to (GGA TCC GTC)
  • GenBank ID
  • Entrez Gene
    CDKN1B (a.k.a. CDKN4, KIP1, MEN1B, MEN4, P27KIP1)
  • Tag / Fusion Protein
    • Luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site Nco I (not destroyed)
  • 5′ sequencing primer GLprimer2 (Promega)
  • (Common Sequencing Primers)

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-p27 T187A Luc was a gift from William Kaelin (Addgene plasmid # 23048 ; ; RRID:Addgene_23048)
  • For your References section:

    Bioluminescent imaging of Cdk2 inhibition in vivo. Zhang GJ, Safran M, Wei W, Sorensen E, Lassota P, Zhelev N, Neuberg DS, Shapiro G, Kaelin WG. Nat Med. 2004 Jun . 10(6):643-8. 10.1038/nm1047 PubMed 15122251