pGL3-p27 T187A Luc
(Plasmid #23048)

• Depositing Lab: William Kaelin
• Publication: Zhang et al Nat Med. 2004 Jun . 10(6):643-8.

Backbone

• Vector backbone: pGL3-control
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5223
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p27
• Alt name: kip1
• Species: H. sapiens (human)
• Insert Size (bp): 607
• Mutation: T187A: mutation at codon 187 changing from T (ACG) to A (GCG), additionally 3 silent mutations introducing a BamH I site at codons 182/183/184 (GGT TCT GTG) to (GGA TCC GTC)
• GenBank ID: U10906
• Entrez Gene: CDKN1B (a.k.a. CDKN4, KIP1, MEN1B, MEN4, P27KIP1)
• Tag / Fusion Protein:
  • Luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind III (not destroyed)
• 3′ cloning site: Nco I (not destroyed)
• 5′ sequencing primer: GLprimer2 (Promega)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry