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pK-Cas9-tracr-L
(Plasmid #230860)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 230860 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pRGEB32
  • Backbone manufacturer
    Yinong Yang
  • Modifications to backbone
    gRNA scaffold was replaced with tracr-L
  • Vector type
    Plant Expression, CRISPR
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Long tracrRNA (tracr-L) from S. pyogenes replaces gRNA scaffold
  • gRNA/shRNA sequence
    20 bp spacer (guide) can be cloned with Bsa1 upstream of tracr-L to target plant genomic locus
  • Promoter OsU3

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pK-Cas9-tracr-L was a gift from Kutubuddin Molla (Addgene plasmid # 230860 ; http://n2t.net/addgene:230860 ; RRID:Addgene_230860)

  • For your References section:

    Adaptation of bacterial natural single guide RNA (tracr-L) for efficient plant genome editing. Karmakar S, Panda D, Behera D, Saha R, Baig MJ, Molla KA. Plant Cell Rep. 2024 Nov 23;43(12):291. doi: 10.1007/s00299-024-03371-z. 10.1007/s00299-024-03371-z PubMed 39579214
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