Adaptation of bacterial natural single guide RNA (tracr-L) for efficient plant genome editing.
Karmakar S, Panda D, Behera D, Saha R, Baig MJ, Molla KA
Plant Cell Rep. 2024 Nov 23;43(12):291. doi: 10.1007/s00299-024-03371-z.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 230860 | pK-Cas9-tracr-L | Long tracrRNA (tracr-L) from Streptococcus pyogenes which acts as a natural single guide RNA |
| 230861 | pK-Cas9-Δtracr-L | A truncated version of tracr-L. A 79 nt in the upper stem extension of tracr-L is replaced with 5'-GAAA-3' to generate Δtracr-L. |