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(Plasmid #23156)


Item Catalog # Description Quantity Price (USD)
Plasmid 23156 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    p817 (pBSII derivate)
  • Vector type
    multicloning site flanked by I-SceI sites

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
  • Copy number
    High Copy


  • Gene/Insert name
    mouse gammaF crystallin promoter:EGFP
  • Alt name
  • Species
    M. musculus (mouse); mouse gammaF promoter:EGFP
  • Insert Size (bp)
  • Mutation
    mouse gammaF crystallin promoter (-225 - +73) preceeding EGFP-SV40pA
  • Entrez Gene
    eGFP (a.k.a. pPRS3a_01)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site I-SceI (not destroyed)
  • 3′ cloning site I-SceI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The original p817 vector was obtained from Jochen Wittbrodt (University of Heidelberg)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A=>G and G=>A mismatches in gene do not have any functional significance.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p817-mgammaFcry-EGFP was a gift from Zbynek Kozmik (Addgene plasmid # 23156 ; ; RRID:Addgene_23156)
  • For your References section:

    A lens-specific co-injection marker for medaka transgenesis. Vopalensky P, Ruzickova J, Pavlu B, Kozmik Z. Biotechniques. 2010 Mar . 48(3):235-6. 10.2144/000113368 PubMed 20359307