pcDNA FRT to BioID-cDIC1-3xFlag
(Plasmid
#232417)
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PurposeExpresses human dynein subunit DIC1 in mammalian cells.
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Depositing Lab
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Depositing OrganizationUniversity of California, San Diego (UCSD)
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 232417 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 232417-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 232417-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA5
- Backbone size w/o insert (bp) 9279
- Total vector size (bp) 11161
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDynein Intermediate Chain 1
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Alt nameDIC1
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SpeciesH. sapiens (human)
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Insert Size (bp)1836
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GenBank IDBC091498.1
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Entrez GeneDYNC1I1 (a.k.a. DNCI1, DNCIC1)
- Promoter CMV
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Tags
/ Fusion Proteins
- BioID (N terminal on insert)
- Flag-3x (C terminal on insert)
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 232417-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 232417-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA FRT to BioID-cDIC1-3xFlag was a gift from Samara Reck-Peterson (Addgene plasmid # 232417 ; http://n2t.net/addgene:232417 ; RRID:Addgene_232417) -
For your References section:
The human cytoplasmic dynein interactome reveals novel activators of motility. Redwine WB, DeSantis ME, Hollyer I, Htet ZM, Tran PT, Swanson SK, Florens L, Washburn MP, Reck-Peterson SL. Elife. 2017 Jul 18;6:e28257. doi: 10.7554/eLife.28257. 10.7554/eLife.28257 PubMed 28718761
Map uploaded by the depositor.
