pcDNA3.1(+)-Flag-SOD1-G93A
(Plasmid
#235662)
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PurposeExpress human SOD1-G93A in mammalian cells
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Depositing Lab
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Depositing OrganizationMonash University, Sunway Campus
Located in Malaysia -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 235662 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 235662-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 235662-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA3.1(+)
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSOD1-G93A
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SpeciesH. sapiens (human)
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Insert Size (bp)579
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Mutationchanged Glycine 93 to alanine
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Entrez GeneSOD1 (a.k.a. ALS, ALS1, HEL-S-44, IPOA, SOD, STAHP, hSod1, homodimer)
- Promoter CMV promoter
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Tag
/ Fusion Protein
- Flag-tag at N-terminal
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer 5-TAATACGACTCACTATAGG-3
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 235662-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 235662-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1(+)-Flag-SOD1-G93A was a gift from Chen Seng Ng (Addgene plasmid # 235662 ; http://n2t.net/addgene:235662 ; RRID:Addgene_235662) -
For your References section:
Integrated transcriptomic profiling reveals a STING-mediated Type II Interferon signature in SOD1-mutant amyotrophic lateral sclerosis models. Hiew JY, Lim YS, Liu H, Ng CS. Commun Biol. 2025 Mar 2;8(1):347. doi: 10.1038/s42003-025-07790-w. 10.1038/s42003-025-07790-w PubMed 40025162
Map uploaded by the depositor.
