pCS2-Opto-Acvr1ba-P2A-Acvr2ba
(Plasmid
#232640)
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PurposeOptogenetic Nodal activators from Sako et al. 2016 (Cell Reports) separated by a P2A peptide for singe-transcript expression
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Depositing Lab
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Depositing OrganizationNational Institute of Child Health and Human Development (NICHD)
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 232640 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 232640-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 232640-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4095
- Total vector size (bp) 7437
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAcvr1ba + VfLOV domain and Acvr2ba + VfLOV domain
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SpeciesD. rerio (zebrafish); Vaucheria frigida
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Insert Size (bp)1893
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Based on the constructs first developed by Sako et al. 2016 (Cell Reports, https://doi.org/10.1016/j.celrep.2016.06.036)
Please visit https://doi.org/10.1101/2025.04.17.649426 for bioRxiv preprint.
DNA (Catalog # 232640-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 232640-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2-Opto-Acvr1ba-P2A-Acvr2ba was a gift from Katherine Rogers (Addgene plasmid # 232640 ; http://n2t.net/addgene:232640 ; RRID:Addgene_232640) -
For your References section:
An optogenetic toolkit for robust activation of FGF, BMP, & Nodal signaling in zebrafish. Iannucci LE, Maria Thomas VS, Anderson WK, Murphy MR, Donahue CET, Campbell CE, Monaghan MT, Saul AJ, Rogers KW. Dev Biol. 2026 Aug;536:155-171. doi: 10.1016/j.ydbio.2026.05.003. Epub 2026 May 18. 10.1016/j.ydbio.2026.05.003 PubMed 42155877
Map uploaded by the depositor.
