PLK3 1-334-pEGFP
(Plasmid #23266)

• Depositing Lab: Wei Dai
• Publication: Yang et al Cancer Res. 2008 Jun 1. 68(11):4077-85.

Backbone

• Vector backbone: pEGFP-N3
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PLK3
• Alt name: CNK
• Alt name: FNK
• Alt name: PRK
• Species: H. sapiens (human)
• Insert Size (bp): 1938
• Mutation: aa 1-334
• Entrez Gene: PLK3 (a.k.a. CNK, FNK, PLK-3, PRK)
• Tag / Fusion Protein:
  • pEGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP-N

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that due to the presence of a 6xHis tag this plasmid appears to encode aa 2-327 for PLK3.