pDsRed1-CMV-Rat Npas4-DsRed1-pA
(Plasmid
#233272)
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PurposeTo Express a Rat NPAS4-DsRed fusion protein from a CMV promoter
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Depositing Labs
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Depositing OrganizationYale University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 233272 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 233272-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 233272-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepdsRedN-N1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namerat Npas4 DsRed fusion
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SpeciesR. norvegicus (rat)
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MutationY2D
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Entrez GeneNpas4 (a.k.a. Nxf)
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Tag
/ Fusion Protein
- DsRed1 (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Be advised the NPAS4 coding region contains a Y2D mutation
DNA (Catalog # 233272-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 233272-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDsRed1-CMV-Rat Npas4-DsRed1-pA was a gift from Jonathan Ploski & Glenn Schafe (Addgene plasmid # 233272 ; http://n2t.net/addgene:233272 ; RRID:Addgene_233272) -
For your References section:
The neuronal PAS domain protein 4 (Npas4) is required for new and reactivated fear memories. Ploski JE, Monsey MS, Nguyen T, DiLeone RJ, Schafe GE. PLoS One. 2011;6(8):e23760. doi: 10.1371/journal.pone.0023760. Epub 2011 Aug 22. 10.1371/journal.pone.0023760 PubMed 21887312
