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pT3TS-Hf-RfxCas13d-HA
(Plasmid #234479)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234479 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 234479-D50 50 μg of DNA in Tris buffer $495
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DNA 234479-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pT3TS
  • Total vector size (bp) 6115
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RfxCas13d
  • Alt name
    CasRx
  • Species
    Ruminococcus flavefaciens XPD3002
  • Insert Size (bp)
    2934
  • Mutation
    Changed four alanine to valine in positions 134, 140, 141 and 143 (A134V, A140V, A141V and A143V)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Addgene plasmid #141320 used as template

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Point mutations performed as described in Tong et al., 2023 (Nat Bio) to generate a High-Fidelity version of RfxCas13d (Hf-RfxCas13d).

Please visit https://doi.org/10.1101/2024.10.08.617220 for bioRxiv preprint.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT3TS-Hf-RfxCas13d-HA was a gift from Miguel Angel Moreno-Mateos (Addgene plasmid # 234479 ; http://n2t.net/addgene:234479 ; RRID:Addgene_234479)

  • For your References section:

    Enhanced RNA-targeting CRISPR-Cas technology in zebrafish. Moreno-Sanchez I, Hernandez-Huertas L, Nahon-Cano D, Martinez-Garcia PM, Treichel AJ, Gomez-Marin C, Tomas-Gallardo L, da Silva Pescador G, Kushawah G, Egidy R, Perera A, Diaz-Moscoso A, Cano-Ruiz A, Walker JA 2nd, Munoz MJ, Holden K, Galceran J, Nieto MA, Bazzini AA, Moreno-Mateos MA. Nat Commun. 2025 Mar 16;16(1):2591. doi: 10.1038/s41467-025-57792-9. 10.1038/s41467-025-57792-9 PubMed 40091120
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