pOT020
(Plasmid
#234943)
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PurposeConstitutive expression of synaptophysin linked to Gamillus fluorescent protein
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Depositing Lab
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Depositing OrganizationUniversity of California, San Francisco (UCSF)
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 234943 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneLenti CAG
- Total vector size (bp) 10000
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSynaptophysin-Gamillus
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Alt nameSYP
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SpeciesH. sapiens (human)
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Tag
/ Fusion Protein
- Fusion
Resource Information
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A portion of this plasmid was derived from a plasmid made byThomas Oertner (Addgene plasmid # 119738, SynTagMA_pre), Takeharu Nagai (Addgene plasmid # 124837, Gamillus/pcDNA3), and Martin Kampmann (Addgene plasmid # 133060, pJC49).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOT020 was a gift from Martin Kampmann (Addgene plasmid # 234943 ; http://n2t.net/addgene:234943 ; RRID:Addgene_234943) -
For your References section:
CRISPRi-based screen of autism spectrum disorder risk genes in microglia uncovers roles of ADNP in microglia endocytosis and synaptic pruning. Teter OM, McQuade A, Hagan V, Liang W, Drager NM, Sattler SM, Holmes BB, Castillo VC, Papakis V, Leng K, Boggess S, Nowakowski TJ, Wells J, Kampmann M. Mol Psychiatry. 2025 Sep;30(9):4176-4193. doi: 10.1038/s41380-025-02997-z. Epub 2025 Apr 6. 10.1038/s41380-025-02997-z PubMed 40188316