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CRISPRi-based screen of autism spectrum disorder risk genes in microglia uncovers roles of ADNP in microglia endocytosis and synaptic pruning.

Teter OM, McQuade A, Hagan V, Liang W, Drager NM, Sattler SM, Holmes BB, Castillo VC, Papakis V, Leng K, Boggess S, Nowakowski TJ, Wells J, Kampmann M
Mol Psychiatry. 2025 Sep;30(9):4176-4193. doi: 10.1038/s41380-025-02997-z. Epub 2025 Apr 6. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
234942pOT056Constitutive expression of Gamillus fluorescent protein
234943pOT020Constitutive expression of synaptophysin linked to Gamillus fluorescent protein
234944pKL078Constitutive expression of membrane receptor Lck linked to mNeonGreen fluorescent protein and a nuclear localized mTagBFP2 fluorescent protein

Antibodies from Article