UbC Puro ABL FRET P112L
(Plasmid
#235034)
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PurposeExpression of BCR-ABL P112L FRET biosensor
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 235034 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepUltra
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Vector typeLentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBCR-ABL with internal StayGold and mScarlet3 tags
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SpeciesH. sapiens (human)
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MutationP112L
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Entrez GeneABL1 (a.k.a. ABL, BCR-ABL, CHDSKM, JTK7, bcr/abl, c-ABL, c-ABL1, p150, v-abl)
- Promoter human ubiquitin C
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Tag
/ Fusion Protein
- PuroR-P2A (N terminal on insert)
Cloning Information
- Cloning method Gene Synthesis
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
mStayGold and mScarlet3 FRET pair measure ABL conformation
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
UbC Puro ABL FRET P112L was a gift from Justin Pritchard (Addgene plasmid # 235034 ; http://n2t.net/addgene:235034 ; RRID:Addgene_235034) -
For your References section:
Dual functional genomics reveals a broad and convergent landscape of asciminib resistance in BCR::ABL1. Sokirniy I, Pritchard J. Genome Med. 2026