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Lenti ccdB sgRNA Hyg sfGFP
(Plasmid #235048)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235048 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    lenti-sgRNA hygro (Addgene Plasmid #104991)
  • Backbone manufacturer
    Brett Stringer
  • Vector type
    Lentiviral, CRISPR
  • Selectable markers
    Hygromycin ; sfGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Adapted from lenti-sgRNA hygro (Plasmid #104991). Requires ccdB resistant competent cells

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lenti ccdB sgRNA Hyg sfGFP was a gift from Justin Pritchard (Addgene plasmid # 235048 ; http://n2t.net/addgene:235048 ; RRID:Addgene_235048)

  • For your References section:

    Dual functional genomics reveals a broad and convergent landscape of asciminib resistance in BCR::ABL1. Sokirniy I, Pritchard J. Genome Med. 2026
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