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UbC SHP2 E76G FRET
(Plasmid #235050)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235050 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pUltra
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SHP2 with internal StayGold and mScarlet3 tags
  • Alt name
    PTPN11
  • Species
    H. sapiens (human)
  • Mutation
    E76G
  • Entrez Gene
    PTPN11 (a.k.a. BPTP3, CFC, JMML, METCDS, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2)
  • Promoter human ubiquitin C

Cloning Information

  • Cloning method Gene Synthesis

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

mStayGold and mScarlet3 FRET pair measure SHP2 conformation

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UbC SHP2 E76G FRET was a gift from Justin Pritchard (Addgene plasmid # 235050 ; http://n2t.net/addgene:235050 ; RRID:Addgene_235050)

  • For your References section:

    Dual functional genomics reveals a broad and convergent landscape of asciminib resistance in BCR::ABL1. Sokirniy I, Pritchard J. Genome Med. 2026
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