Ef1a-NBALFA-NotchGal4VP64-T2A-mCherry (pSS038)
(Plasmid
#236340)
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PurposeMTK transcriptional unit vector encoding an NbALFA-synNotch and an mCherry transduction marker
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 236340 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepMK1213 LS/RE Lentiviral Adapter Vector
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEf1a-NBALFA-NotchGal4VP64-T2A-mCherry
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SpeciesSynthetic
- Promoter Ef1a
Cloning Information
- Cloning method Golden Gate
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.03.12.584464 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Ef1a-NBALFA-NotchGal4VP64-T2A-mCherry (pSS038) was a gift from Hana El-Samad (Addgene plasmid # 236340 ; http://n2t.net/addgene:236340 ; RRID:Addgene_236340)