Hana El-Samad Lab: Small molecule and cell contact-inducible systems for controlling expression and differentiation in stem cells
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 236336 | EF1a-NBALFA-Notch361-T2A-mCherry (pSS032) | MTK transcriptional unit vector encoding an NbALFA-SNIPR and an mCherry transduction marker |
| 236337 | EF1a-ALFAfibcon-PDGFR-T2A-mCherry (pSS034) | MTK transcriptional unit vector encoding an ALFA fibcon ligand with PDGFRb transmembrane domain and an mCherry transduction marker |
| 236338 | EF1a-NBALFA-Notch361-T2A-mCherry (pSS035) | Second generation lentivirus compatible transfer vector encoding an NbALFA-SNIPR and an mCherry transduction marker |
| 236339 | EF1a-ALFAfibcon-PDGFRb-T2A-mCherry (pSS037) | Second generation lentivirus compatible transfer vector encoding an ALFA fibcon ligand with PDGFRb transmembrane domain and an mCherry transduction marker |
| 236340 | Ef1a-NBALFA-NotchGal4VP64-T2A-mCherry (pSS038) | MTK transcriptional unit vector encoding an NbALFA-synNotch and an mCherry transduction marker |
| 236341 | Ef1a-NBALFA-NotchGal4VP64-T2A-mCherry (pSS039) | Second generation lentivirus compatible transfer vector encoding NbALFA-synNotch and an mCherry transduction marker |
| 236342 | Gal4UAS_YBTATA_Ngn2_PGK_tagBFP_WPRE (pSS051) | 2nd gen LV compatible transfer vector encoding an inducible Ngn2 downstream of the Gal4 upstream activating seq and ybTATA minimal promoter along with a PGK promoter-drive TagBFP transduction marker |
| 236343 | Ef1a-Gal4-VP64-Ert2 (pSS052) | MTK transcriptional unit vector encoding ERT2 expression system |
| 236345 | Ef1a-VP64-NS3-Gal4_DBD (pSS054) | MTK transcriptional unit vector encoding NS3 expression system |
| 236346 | pPGK-mCherry-Empty (pSS055) | MTK transcriptional unit vector encoding mCherry transduction marker |
| 236347 | Ef1a-Gal4-VP64-Ert2-pPGK-mCherry (pSS056) | Second generation lentivirus compatible transfer vector encoding an ERT2 gene expression system and an mCherry transduction marker |
| 236348 | Ef1a-VP16-PYL-T2A-GAL4_DBD-ABI-pPGK-mCherry (pSS057) | Second generation lentivirus compatible transfer vector encoding an NS3 gene expression system and an mCherry transduction marker |
| 236349 | Ef1a-VP64-NS3-Gal4_DBD-pPGK-mCherry (pSS058) | Second generation lentivirus compatible transfer vector encoding an ABI gene expression system and an mCherry transduction marker |
| 236350 | EF1a-TetOn3G-T2A-mCherry (pSS059) | MTK transcriptional unit vector encoding the TetOn3G transcription factor and an mCherry transduction marker |
| 236351 | EF1a-TetOn3G-T2A-mCherry (pSS060) | Second generation lentivirus compatible transfer vector encoding the TetOn3G transcription factor and an mCherry transduction marker |
| 236353 | Ef1a-NLS-Gal4VP64TF-T2A-mCherry (MC) (pSS085) | Second generation lentivirus compatible transfer vector encoding an GAL4 and an mCherry transduction marker |
| 236354 | ALFA-Fibcon (pZYW250) | Encodes ALFA epitope tag fused to a single fibcon domain as a part 3A to be used in the MTK |
| 236355 | pTET-Ngn2::pPGK-TagBFP (pZYW457) | Ngn2 driven by a Tetracycline Responsive Element, with a TagBFP transduction marker. Derived from pSS051 |
| 236356 | pTET-TagBFP::pPGK-mCitrine (pZYW458) | TagBFP driven by a Tetracycline Responsive Element, with an mCitrine transduction marker. Derived from pRL407 |
| 236357 | NBALFA (pDS1188) | Anti-ALFA tag nanobody to be used as a part 3A in the MTK |
| 236358 | GAL4-VP64-ERT2 (pAN3541) | Encodes ERT2 gene expression system as a Type 3 part to be used in the MTK system |
| 236359 | VP64-NS3 (pAN3950) | Encodes NS3 gene expression system as a Type 3A part to be used in the MTK system |
| 236360 | GAL4 DBD (pAN029) | Encodes GAL4 DNA binding domain to be used as a Type 3B in the MTK system |