Nluc-YAP1_TEAD4-HaloTag Bidirectional(Balanced) Vector
(Plasmid #237003)

• Purpose: Express NLuc-YAP1_TEAD4-HaloTag(R) Fusion Proteins in Mammalian Cells under a CMV promoter
• Depositing Lab: Promega Corporation
• Depositing Organization: Promega Corporation
  Located in United States of America
• Publication: Promega Plasmid Collection (unpublished)

Backbone

• Vector backbone: pFN224C NanoLuc(R) CMV-Neo BiBRET-Balanced Vector and pFC221K HaloTag(R) CMV-Blast BiBRET Vector
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: YAP1
• Species: H. sapiens (human)
• Mutation: None
• Entrez Gene: YAP1 (a.k.a. COB1, YAP, YAP-1, YAP2, YAP65, YKI)
• Promoter: CMV
• Tag / Fusion Protein:
  • NanoLuc (R) (N terminal on backbone)

Cloning Information for Gene/Insert 1

• Cloning method: Other

Gene/Insert 2

• Gene/Insert name: TEAD4
• Species: H. sapiens (human)
• Entrez Gene: TEAD4 (a.k.a. EFTR-2, RTEF1, TCF13L1, TEF-3, TEF3, TEFR-1, hRTEF-1B)
• Tag / Fusion Protein:
  • HaloTag (R) (C terminal on backbone)

Terms and Licenses

• Academic/Nonprofit Terms:
  • Research Use Only Terms (Nonprofits)
  • HaloTag Vector Limited Use Label License
  • NanoLuc Luciferase Limited Use Label License
  • Promega Contract Research Organization Limited Use Label License
• Industry Terms:
  • Research Use Only Terms (Industry)
  • HaloTag Vector Limited Use Label License
  • NanoLuc Luciferase Limited Use Label License
  • Promega Contract Research Organization Limited Use Label License

DNA (Catalog # 237003-D20)

Purpose

Ready-to-use DNA prep. DNA aliquots are suitable for use in molecular biology and scientific research.

Delivery

• Amount: 20 μg
• Concentration: 1 μg/μL
• Pricing: $413 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • Research Use Only Terms (Nonprofits)
  • HaloTag Vector Limited Use Label License
  • NanoLuc Luciferase Limited Use Label License
  • Promega Contract Research Organization Limited Use Label License
• Industry Terms:
  • Research Use Only Terms (Industry)
  • HaloTag Vector Limited Use Label License
  • NanoLuc Luciferase Limited Use Label License
  • Promega Contract Research Organization Limited Use Label License

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Addgene does not test for endotoxin levels on these samples.