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G790A_AttB_ACE2-miRFP670_IRES_mCherry-H2A-P2A-PuroR
(Plasmid #237440)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 237440 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    Custom
  • Total vector size (bp) 9121
  • Vector type
    Mammalian Expression, Synthetic Biology ; Promotorless Bxb1 DNA recombination
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ACE2
  • Alt name
    Angiotensin-converting enzyme 2
  • Alt name
    hACE2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2445
  • Entrez Gene
    ACE2 (a.k.a. ACEH)
  • Tags / Fusion Proteins
    • miRFP670 (C terminal on insert)
    • mCherry (C terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AACGACGGCCAGTGAATTG
  • 3′ sequencing primer CGTTACTGGCCGAAGCCGC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    G790A_AttB_ACE2-miRFP670_IRES_mCherry-H2A-P2A-PuroR was a gift from Kenneth Matreyek (Addgene plasmid # 237440 ; http://n2t.net/addgene:237440 ; RRID:Addgene_237440)

  • For your References section:

    Kozak sequence libraries for systematically characterizing transgenes across expression levels. Shukla N, Kamath ND, Snell JC, Bruchez AM, Matreyek KA. Nucleic Acids Res. 2026 Jul 17;54(14):gkag728. doi: 10.1093/nar/gkag728. 10.1093/nar/gkag728 PubMed 42475684
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