Kozak sequence libraries for systematically characterizing transgenes across expression levels.

Shukla N, Kamath ND, Snell JC, Bruchez AM, Matreyek KA
Nucleic Acids Res. 2026 Jul 17;54(14):gkag728. doi: 10.1093/nar/gkag728. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
237440	G790A_AttB_ACE2-miRFP670_IRES_mCherry-H2A-P2A-PuroR	Plasmid for strong human ACE2 expression from the Bxb1 landing pad, with a C-terminal miRFP670 fusion includes IRES-driven mCherry-H2A and P2A-linked PuroR for selection and visualization.
237441	G868A_AttB_[mutATG]ACE2-miRFP670_IRES_mCherry-H2A-P2A-PuroR	Plasmid encoding the human ACE2 protein with a C-terminal miRFP670 fusion, designed to create a degenerate ACE2 Kozak library for assessing ACE2-miRFP670 translation rate and steady-state abundance.