G868A_AttB_[mutATG]ACE2-miRFP670_IRES_mCherry-H2A-P2A-PuroR
(Plasmid #237441)

• Purpose: Plasmid encoding the human ACE2 protein with a C-terminal miRFP670 fusion, designed to create a degenerate ACE2 Kozak library for assessing ACE2-miRFP670 translation rate and steady-state abundance.
• Depositing Lab: Kenneth Matreyek
• Publication: Shukla et al Nucleic Acids Res. 2026 Jul 17;54(14):gkag728. doi: 10.1093/nar/gkag728.

Backbone

• Vector backbone: custom
• Backbone size w/o insert (bp): 6670
• Total vector size (bp): 9112
• Vector type: Mammalian Expression, Synthetic Biology ; promoterless Bxb1 recombination vector
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ACE2
• Alt name: Angiotensin-converting enzyme 2
• Alt name: hACE2
• Species: H. sapiens (human)
• Insert Size (bp): 2442
• Mutation: deleted 6 Kozak nucleotides and start codon ATG
• Entrez Gene: ACE2 (a.k.a. ACEH)
• Tags / Fusion Proteins:
  • miRFP670 (C terminal on insert)
  • mCherry (C terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: AACGACGGCCAGTGAATTG
• 3′ sequencing primer: CGTTACTGGCCGAAGCCGC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry