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pACEMam2-TRPML1
(Plasmid #238303)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238303 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 238303-D50 50 μg of DNA in Tris buffer $495
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DNA 238303-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pACEMam2
  • Backbone manufacturer
    Geneva Biotech
  • Backbone size w/o insert (bp) 4618
  • Total vector size (bp) 6484
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRPML1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1887
  • Entrez Gene
    MCOLN1 (a.k.a. LECD, MG-2, ML1, ML4, MLIV, MST080, MSTP080, TRP-ML1, TRPM-L1, TRPML1)
  • Promoter hybrid CAG promoter
  • Tag / Fusion Protein
    • 8xHis-3xFLAG-TEV (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MreI (unknown if destroyed)
  • 3′ cloning site XmnI (unknown if destroyed)
  • 5′ sequencing primer GGCGGGGTTCGGCTTCTG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACEMam2-TRPML1 was a gift from Pravin Mahajan (Addgene plasmid # 238303 ; http://n2t.net/addgene:238303 ; RRID:Addgene_238303)

  • For your References section:

    High throughput cryo-EM provides structural understanding for modulators of the lysosomal ion channel TRPML1. Reeks J, Mahajan P, Clark M, Cowan SR, Di Daniel E, Earl CP, Fisher S, Holvey RS, Jackson SM, Lloyd-Evans E, Morgillo CM, Mortenson PN, O'Reilly M, Richardson CJ, Schopf P, Tams DM, Waller-Evans H, Ward SE, Whibley S, Williams PA, Johnson CN. Structure. 2025 Aug 7;33(8):1374-1385.e7. doi: 10.1016/j.str.2025.05.014. Epub 2025 Jun 17. 10.1016/j.str.2025.05.014 PubMed 40532704
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