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mCherry-TOP1-GFP-pEGFP-N1
(Plasmid #238377)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238377 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pmCherry-EGFP (Addgene #86639)
  • Backbone size w/o insert (bp) 5437
  • Total vector size (bp) 7726
  • Modifications to backbone
    Insertion of Top1 cDNA in KpnI/BamHI sites
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DNA Topoisomerase 1
  • Alt name
    TOP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2295
  • Entrez Gene
    TOP1 (a.k.a. TOPI)
  • Promoter CMV
  • Tags / Fusion Proteins
    • mCherry (N terminal on backbone)
    • eGFP (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-TOP1-GFP-pEGFP-N1 was a gift from Kristijan Ramadan (Addgene plasmid # 238377 ; http://n2t.net/addgene:238377 ; RRID:Addgene_238377)

  • For your References section:

    TEX264 drives selective autophagy of DNA lesions to promote DNA repair and cell survival. Lascaux P, Hoslett G, Tribble S, Trugenberger C, Anticevic I, Otten C, Torrecilla I, Koukouravas S, Zhao Y, Yang H, Aljarbou F, Ruggiano A, Song W, Peron C, Deangeli G, Domingo E, Bancroft J, Carrique L, Johnson E, Vendrell I, Fischer R, Ng AWT, Ngeow J, D'Angiolella V, Raimundo N, Maughan T, Popovic M, Milosevic I, Ramadan K. Cell. 2024 Oct 3;187(20):5698-5718.e26. doi: 10.1016/j.cell.2024.08.020. Epub 2024 Sep 11. 10.1016/j.cell.2024.08.020 PubMed 39265577
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