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NanoBRET Assay Vector, BiBRET CRAF-BRAF
(Plasmid #238574)

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Item Catalog # Description Quantity Price (USD)
Plasmid 238574 Standard format: Plasmid sent in bacteria as agar stab 1 $94 *
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DNA 238574-D20 20 μg of DNA in Tris buffer $413 *
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DNA 238574-D50 50 μg of DNA in Tris buffer $495
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DNA 238574-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pFC221K HaloTag(R) CMV-Blast BiBRET Vector and pFN224C NanoLuc(R) CMV-Neo BiBRET-Balanced Vector
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    CRAF
  • Species
    H. sapiens (human)
  • Mutation
    None
  • Entrez Gene
    RAF1 (a.k.a. CMD1NN, CRAF, NS5, Raf-1, c-Raf)
  • Promoter CMV
  • Tag / Fusion Protein
    • NanoLuc (R) (N terminal on backbone)

Cloning Information for Gene/Insert 1

  • Cloning method Other

Gene/Insert 2

  • Gene/Insert name
    BRAF
  • Species
    H. sapiens (human)
  • Entrez Gene
    BRAF (a.k.a. B-RAF1, B-raf, BRAF-1, BRAF1, NS7, RAFB1)
  • Tag / Fusion Protein
    • HaloTag (R) (C terminal on backbone)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use DNA prep. DNA aliquots are suitable for use in molecular biology and scientific research.

Delivery

  • Amount 20 μg
  • Concentration 1 μg/μL
  • Pricing $413 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Addgene does not test for endotoxin levels on these samples.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NanoBRET Assay Vector, BiBRET CRAF-BRAF was a gift from Promega Corporation (Addgene plasmid # 238574 ; http://n2t.net/addgene:238574 ; RRID:Addgene_238574)
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