pCAGGS_hTRPV1-p2A-tdTomato
(Plasmid
#238923)
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PurposeExpression of the human TRPV1 channel with tdTomato as a reporter in mammalian cells
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Depositing Lab
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Depositing OrganizationShemyakin-Ovchinnikov Institute of Bioorganic Chemistry (INACTIVE)
Located in Russian Federation -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 238923 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 238923-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 238923-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCAG_PSD95.FingR-eGFP-CCR5TC
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Backbone manufacturerDon Arnold (Addgene #46295)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehTRPV1-p2A-tdTomato
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SpeciesSynthetic
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Insert Size (bp)4116
- Promoter CAG
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 238923-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 238923-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAGGS_hTRPV1-p2A-tdTomato was a gift from Vsevolod Belousov (Addgene plasmid # 238923 ; http://n2t.net/addgene:238923 ; RRID:Addgene_238923) -
For your References section:
Human TRPV1 is an efficient thermogenetic actuator for chronic neuromodulation. Maltsev DI, Solotenkov MA, Mukhametshina LF, Sokolov RA, Solius GM, Jappy D, Tsopina AS, Fedotov IV, Lanin AA, Fedotov AB, Krut' VG, Ermakova YG, Moshchenko AA, Rozov A, Zheltikov AM, Podgorny OV, Belousov VV. Cell Mol Life Sci. 2024 Oct 25;81(1):437. doi: 10.1007/s00018-024-05475-x. 10.1007/s00018-024-05475-x PubMed 39448456
